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From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila

Zipursky, S. L. and Venkatesh, T. R. and Benzer, Seymour (1985) From monoclonal antibody to gene for a neuron-specific glycoprotein in Drosophila. Proceedings of the National Academy of Sciences of the United States of America, 82 (6). pp. 1855-1859. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ZIPpnas85

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Abstract

A monoclonal antibody (MAb24B10), derived from mice immunized with Drosophila retina, exclusively stains photoreceptor cells in the retina and their axonal projections to the optic ganglia. The antigen (Ag24B10) is a 160-kDa glycoprotein comprising about 0.8% of the retina protein. By microsequencing, 19 of the first 21 amino acids at the NH2-terminal end of the protein have been determined. Using synthetic oligonucleotide probes corresponding to a portion of this amino acid sequence, we isolated a homologous genomic clone. A partial DNA sequence of this clone, along with blot experiments on genomic DNA and RNA, indicate that this clone is part of the structural gene for Ag24B10. By in situ hybridization, the gene was localized to the tip of chromosome 3R.


Item Type:Article
Additional Information:© 1985 by the National Academy of Sciences. Contributed by Seymour Benzer, November 8, 1984. We thank Drs. Dennis Ballinger, Elliot Meyerowitz, and David Teplow for helpful discussion and, in particular, Dr. Meyerowitz for generously providing laboratory facilities. This work was supported by the Helen Hay Whitney Foundation (S.L.Z.), the Gosney Foundation (T.R.V.), and National Science Foundation Grant PCM 79-11771 (S.B.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:neurodevelopment; retina; oligonucleotide probes; in situ chromosomal location
Record Number:CaltechAUTHORS:ZIPpnas85
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ZIPpnas85
Alternative URL:http://www.pnas.org/cgi/content/abstract/82/6/1855
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10078
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Apr 2008
Last Modified:26 Dec 2012 09:56

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