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Reduction of caveolin and caveolae in oncogenically transformed cells

Koleske, Anthony J. and Baltimore, David and Lisanti, Michael P. (1995) Reduction of caveolin and caveolae in oncogenically transformed cells. Proceedings of the National Academy of Sciences of the United States of America, 92 (5). pp. 1381-1385. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:KOLpnas95

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Abstract

Caveolae are flask-shaped non-clathrin-coated invaginations of the plasma membrane. In addition to the demonstrated roles for caveolae in potocytosis and transcytosis, caveolae may regulate the transduction of signals from the plasma membrane. Transformation of NIH 3T3 cells by various oncogenes leads to reductions in cellular levels of caveolin, a principal component of the protein coat of caveolae. The reduction in caveolin correlates very well with the size of colonies formed by these transformed cells when groan in soft agar. Electron microscopy reveals that caveolae are morphologically absent from these transformed cell lines. These observations suggest that functional alterations in caveolae may play a critical role in oncogenic transformation, perhaps by disrupting contact inhibition in transformed cells.


Item Type:Article
Additional Information:Copyright © 1995 by the National Academy of Sciences. Contributed by David Baltimore, November 28, 1994. We thank Ya-Huei Tu for her expert assistance in electron microscopy. We thank Drs. R. O. Hynes and K. Alexandropoulos for critical review of this manuscript. A.J.K. thanks the members of the Baltimore and Lisanti labs for many helpful discussions. This work was aided by a postdoctoral fellowship from the Jane Coffin Childs Memorial Fund for Medical Research to A.J.K. and by a grant from the W. M. Keck Foundation supporting the Whitehead Fellows Program to M.P.L. The research was supported by National Institutes of Health Grants GM-50443 to M.P.L. and CA-51462 to D.B. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:cancer, growth regulation, oncogene, potocytosis, rous-sarcoma virus, malignant transformation, tyrosine phosphorylation, protein-component, growth, permeability, fibroblasts, capillaries, inhibition, transport
Record Number:CaltechAUTHORS:KOLpnas95
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KOLpnas95
Alternative URL:http://www.pnas.org/cgi/content/abstract/92/5/1381
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1011
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Dec 2005
Last Modified:26 Dec 2012 08:42

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