Citri, Yoav and Braun, Jonathan and Baltimore, David (1987) Elevated myc expression and c-myc amplification in spontaneously occurring B lymphoid cell lines. Journal of Experimental Medicine, 165 (4). pp. 1188-1194. ISSN 0022-1007 http://resolver.caltech.edu/CaltechAUTHORS:CITjem87
See Usage Policy.
Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:CITjem87
Recently, a minor subpopulation of murine B lymphocytes, Ly-1+ B cells, has been distinguished by its unique ontogeny, tissue distribution, and prominence in certain autoimmune and neoplastic B cell diseases. We have previously described a simple murine spleen culture system that results in the spontaneous and exclusive outgrowth of long-term Ly-1+ B cell lines (B Ly-1 cells). Here, we report that the immortal growth property of B Ly-1 cells correlates with a 10-45-fold elevation of steady-state myc RNA and 2-10-fold amplification of the c-myc locus. While c-myc amplification has been observed in malignant cell lines derived from several tissues of origin, its occurrence in lymphoid cells has not been previously reported. The consistent c-myc amplification in B Ly-1 cells may reflect a unique state of this locus in the Ly-1+ B lymphocyte lineage, and contribute to the spontaneous immortalization of this B cell population in vitro, and its apparent predilection for malignant transformation in vivo.
|Additional Information:||Copyright © 1987 by Rockefeller University Press. Received for publication 18 December 1986. We thank Dr. Rhona Schreck (Department of Medicine, UCLA) for cytogenetic analysis, Fardad Forouzanpour for laboratory assistance, Debra Otis and Giok Brandt for patient and skilled manuscript preparation, and Carol Appleton for able photographic services.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||14 Apr 2008|
|Last Modified:||26 Dec 2012 09:57|
Repository Staff Only: item control page