Roberts, John W. and Grula, John W. and Posakony, James W. and Hudspeth, Richard and Davidson, Eric H. and Britten, Roy J. (1983) Comparison of sea urchin and human mtDNA: Evolutionary rearrangement. Proceedings of the National Academy of Sciences of the United States of America, 80 (15). pp. 4614-4618. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:ROBpnas83
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Clones of full-length mtDNA have been isolated from a Strongylocentrotus franciscanus recombinant DNA library by secreening a cDNA clone of cytochrome oxidase subunit 1 mRNA. Restriction fragment cross-hybridization analysis shows the following difference in gene arrangement between sea urchin and human mtDNA. The 16S rRNA and cytochrome oxidase subunit 1 genes are directly adjacent in sea urchin mtDNA. These two genes are separated in human and other mammalian mtDNAs by the region containing unidentified reading frames 1 and 2. In spite of the difference in gene order, gene polarity appears to have been conserved. We conclude that the difference in gene order reflects a rearrangement that took place in the sea urchin lineage since sea urchins and mammals last shared a common ancestor.
|Additional Information:||© 1983 by the National Academy of Sciences. Contributed by Roy J. Britten, April 4, 1983. We wish to thank M. King and Dr. G. Attardi for the gift of human mtDNA. We also thank Dr. H. Jacobs for much helpful discussion. This research was supported by National Institutes of Health Grant GM-20927 and by Biomedical Research Support Grant RR-07003. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||recombinant DNA; hybridization; gene organization|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||15 Apr 2008|
|Last Modified:||14 Nov 2014 19:20|
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