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Evidence for altered DNA conformations in the simian virus 40 genome: Site-specific DNA cleavage by the chiral complex Lambda-tris(4,7-diphenyl-1,10-phenanthroline)cobalt(III)

Müller, Barbara C. and Raphael, Adrienne L. and Barton, Jacqueline K. (1987) Evidence for altered DNA conformations in the simian virus 40 genome: Site-specific DNA cleavage by the chiral complex Lambda-tris(4,7-diphenyl-1,10-phenanthroline)cobalt(III). Proceedings of the National Academy of Sciences of the United States of America, 84 (7). pp. 1764-1768. ISSN 0027-8424. PMCID PMC304521.

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Lambda-Tris(4,7-diphenyl-1,10-phenanthroline)cobalt(III), a photoactivated DNA-cleaving agent, is a small molecular probe of DNA structure. Because of its chirality, the complex cannot bind to regular right-handed B-form DNA but exhibits site-specific cleavage along the polymer strand at conformationally distinct sites such as those in a left-handed conformation. Both coarse and higher resolution mapping experiments using the chiral cobalt complex indicate intriguing conformational variations along the simian virus 40 genome. Highly specific cleavage is evident in the enhancer and promoter blocks and in the region downstream of 3' termini. A specific cleavage pattern borders an alternating purine/pyrimidine stretch within the enhancer, which was found earlier to bind anti-Z-DNA antibodies. Throughout the simian virus 40 genome, variations in structure delineated with the cobalt complex appear to correlate with regions important for control of gene expression.

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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1987 National Academy of Sciences. Communicated by Nicholas J. Turro, November 10, 1986 (received for review September 17, 1986). We are grateful for the generous support of the National Institutes of Health (GM33309), the National Foundation for Cancer Research, and (to B.C.M.) the Deutscher Akademischer Austauschdienst and Verband der Chemischen Industrie. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
National Foundation for Cancer ResearchUNSPECIFIED
Deutscher Akademischer Austauschdienst (DAAD)UNSPECIFIED
Verband der Chemischen IndustrieUNSPECIFIED
Subject Keywords:DNA secondary structure, Z-DNA, photoactivated DNA probe, promoter, enhancer
PubMed Central ID:PMC304521
Record Number:CaltechAUTHORS:MULpnas87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1016
Deposited By: Tony Diaz
Deposited On:01 Dec 2005
Last Modified:26 Jan 2016 19:54

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