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Studies on nucleic acid reassociation kinetics: Reactivity of single-stranded tails in DNA-DNA renaturation

Smith, Michael J. and Britten, Roy J. and Davidson, Eric H. (1975) Studies on nucleic acid reassociation kinetics: Reactivity of single-stranded tails in DNA-DNA renaturation. Proceedings of the National Academy of Sciences of the United States of America, 72 (12). pp. 4805-4809. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SMIpnas75

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Abstract

The reassociation kinetics of Escherichia coli DNA were measured by S1 nuclease resistance and hydroxyapatite binding. While the reaction assayed by hydroxyapatite displays second order kinetics, the S1 nuclease measurements follow a non-second order form, as previously reported by Morrow (Ph.D. Dissertation, Stanford University, 1974). Much of the reaction measured with S1 nuclease occurs between single stranded regions of fragments already bearing duplex structures from previous collisions, and between such regions and totally free single strands. Experimental determinations indicate that the nucleation rate of single stranded regions on fragments also containing duplexes is inhibited by an average factor of 2 to 4.


Item Type:Article
Additional Information:© 1975 by the National Academy of Sciences. Contributed by Roy J. Britten, September 15, 1975. We acknowledge the expert technical assistance of Ms. Margaret Chamberlin and Ms. Denise Painchaud. We thank Drs. Norman Davidson and Lynn Klotz for their critical reviews of a draft of this paper. This research was supported by NIH Grants HD-05753 and GM-20927 and by NSF Grant GB-33441.
Subject Keywords:S1 nuclease; hydroxyapatite
Record Number:CaltechAUTHORS:SMIpnas75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SMIpnas75
Alternative URL:http://www.pnas.org/cgi/content/abstract/72/12/4805
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10196
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Apr 2008
Last Modified:26 Dec 2012 09:57

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