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Isolation and structure of pro-somatostatin: A putative somatostatin precursor from pig hypothalamus

Schally, Andrew V. and Huang, Wei-Yong and Chang, Robert C. C. and Arimura, Akira and Redding, Tommie W. and Millar, Robert P. and Hunkapiller, M. W. and Hood, L. E. (1980) Isolation and structure of pro-somatostatin: A putative somatostatin precursor from pig hypothalamus. Proceedings of the National Academy of Sciences of the United States of America, 77 (8). pp. 4489-4493. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:SCHApnas80

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Abstract

An octacosapeptide that we named pro-somatostatin has been isolated from acid extracts of porcine hypothalami and found to have the amino acid sequence Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly- overline{Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys}. This octacosapeptide possesses high somatotropin (growth hormone) and prolactin release-inhibiting activity in vitro. It also crossreacts strongly with antisera generated against the somatostatin tetradecapeptide. This octacosapeptide is most likely a precursor (pro-hormone) of somatostatin in the hypothalamus. The existence of still larger molecular size precursors of somatostatin was also observed.


Item Type:Article
Additional Information:© 1980 by the National Academy of Sciences. Contributed by Andrew V. Schally, April 24, 1980. It is a pleasure to acknowledge the participation of Drs. A. Dupont, H. Matsuo, K. Kangawa, and Raquel Fernandez-Durango in the early stages of this work. This work was supported in part by grants from the Veterans Administration and by U.S. Public Health Service Grants AM-07467 (to A.V.S.), AM-09094 (to A.A.), and GM-06965 (to L.E.H.). R.P.M. was a recipient of a Fellowship from the Medical Research Council of South Africa. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:amino acid sequence; pro-hormone
Record Number:CaltechAUTHORS:SCHApnas80
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:SCHApnas80
Alternative URL:http://www.pnas.org/cgi/content/abstract/77/8/4489
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10218
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Apr 2008
Last Modified:26 Dec 2012 09:58

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