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Markedly different ATP requirements for rRNA synthesis and mtDNA light strand transcription versus mRNA synthesis in isolated human mitochondria

Gaines, George and Rossi, Cesare and Attardi, Giuseppe (1987) Markedly different ATP requirements for rRNA synthesis and mtDNA light strand transcription versus mRNA synthesis in isolated human mitochondria. Journal of Biological Chemistry, 262 (4). pp. 1907-1915. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:GAIjbc87

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Abstract

In isolated mitochondria from HeLa cells, the ATP requirements for mitochondral DNA (mtDNA) transcription and RNA processing can be satisfied by either endogenous synthesis, mainly through oxidative- phosphorylation, or by exogenous supply. The pattern of RNA synthesis changes dramatically depending upon the level of ATP available. At the low intramitochondrial ATP levels produced from endogenous ADP in the presence of an oxidizable substrate and phosphate, the mRNA species are labeled to a substantial extent, whereas there is only a marginal labeling of the rRNA species and light (L) strand transcripts. By contrast, high ATP levels, either provided exogenously or produced endogenously in the presence of an oxidizable substrate, phosphate, and exogenous ADP, strongly stimulate rRNA synthesis (about 10-fold) and light (L) strand transcription (greater than 10-fold), with only a slight increase in mRNA synthesis.


Item Type:Article
Additional Information:Copyright © 1987 by the American Society for Biochemistry and Molecular Biology. (Received for publication, June 18, 1986) We thank Mike King for his generous gifts of the M13 clones used in this study. We also thank Anne Chomyn and Ram S. Puranam for their critical reading of the manuscript. The technical assistance of Arger Drew is gratefully acknowledged. These investigations were supported by Grants GM-11726 and T32 GM-07616 from the National Institutes of Health. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. [C.R. was the] [r]ecipient of a Gosney Fellowship and a Fellowship of the Associazione Italiana per la Ricerca sul Cancro.
Record Number:CaltechAUTHORS:GAIjbc87
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GAIjbc87
Alternative URL:http://www.jbc.org/cgi/content/abstract/262/4/1907
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ID Code:10280
Collection:CaltechAUTHORS
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Deposited On:22 Apr 2008
Last Modified:26 Dec 2012 09:58

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