Fristrom, J. W. and Mitchell, H. K. (1965) The preparative isolation of imaginal discs from larvae of Drosophila melanogaster. Journal of Cell Biology, 27 (2). pp. 445-448. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:FRIjcb65
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Imaginal discs of insccts have long been known to be embryonic Anlagen (1). Indeed, in Drosophila, the external structures in the head and thoracic regions, as well as some in the abdominal region, develop from imaginal discs (2). Discs develop normally when transplanted into larvae, and Schneider (3) has recently shown that eye discs develop normally and become pigmented in a defined medium. Thus, for the study of morphogenesis, imaginal discs offer a convenient tool. However, such studies have previously been hampered by the requirement for tedious manual dissection of discs. The present paper outlines a procedure for isolating large numbers of discs without dissection.
|Additional Information:||Copyright © 1965 by Rockefeller University Press. Received for publication, June 28, 1965. The senior author is a National Science Foundation Postdoctoral Fellow. The investigation is supported in part by grant GB-140 from the National Science Foundation. The authors are indebted to Dr. E.B. Lewis for the photographs, and to Miss Ursula Weber and Mrs. Annamarie Mitchell for technical assistance.|
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|Deposited On:||22 Apr 2008|
|Last Modified:||26 Dec 2012 09:58|
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