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Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone

Kung, Patrick C. and Silverstone, Allen E. and McCaffrey, Ronald P. and Baltimore, David (1975) Murine terminal deoxynucleotidyl transferase: cellular distribution and response to cortisone. Journal of Experimental Medicine, 141 (4). pp. 855-865. ISSN 0022-1007. http://resolver.caltech.edu/CaltechAUTHORS:KUNjem75

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Abstract

The mouse thymus contains two forms of terminal deoxynucleotidyl transferase (TdT) which are distinguishable by the salt concentration necessary to elute them from a phosphocellulose column, by their distrubtion among the thymocyte subpopulations, and by their sensitivity to cortisone treatment. In the whole thymus the later eluting peak (peak II) is the predominant one with about 3-10% of the total activity appearing in peak I. Both peak I and peak II activities are most sensitively assayed by the polymerization of dGMP onto an oligo(dA) primer. The minor population of thymocytes which is less dense and cortisone-resistant contains a higher specific activity of peak I TdT. The majority of TdT activity is, however, found in the major population of thymocytes which occurs in the center region of a bovine serum albumin gradient and is cortisone-sensitive. A very low level of an activity indistinguishable from peak II TdT activity is also detected in the mouse bone marrow. Other tissues, such as spleen, liver, heart, and brain lack detectable amounts of TdT activity.


Item Type:Article
Additional Information:Copyright © 1975 by Rockefeller University Press. Received for publication 23 December 1974. We are very grateful to Dr. Robertson Parkman for his advice on the use of bovine serum albumin gradients, and to Thomas A. Harrison and Donna F. Smoler for their valuable technical assistance. Supported by a contract from the Virus Cancer Program of the National Cancer Institute and grant no. CA-14051 from the National Institutes of Health. Presented in part at the 14th Annual Meeting of the American Society for Cell Biology, San Diego, Calif., November 1974. [P.C.K. was a] Postdoctoral fellow of the Jane Coffin Childs Fund for Medical Research. [A.E.S. was a] Postdoctoral fellow of the Massachusetts Division of the American Cancer Society. [D.B. was an] American Cancer Society Professor of Microbiology.
Record Number:CaltechAUTHORS:KUNjem75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:KUNjem75
Alternative URL:http://www.jem.org/cgi/content/abstract/141/4/855
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10301
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:22 Apr 2008
Last Modified:26 Dec 2012 09:58

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