Xie, J. and Shih, J. and Lee, T. D. and Tai, Y. C. (2005) Complete gradient-LC-ESI system on a chip for protein analysis. In: IEEE International Conference on Micro Electro Mechanical Systems, 18th . MEMS 2005. IEEE , Piscataway, NJ, pp. 778-781. ISBN 0-7803-8732-5 http://resolver.caltech.edu/CaltechAUTHORS:XIEieeeicmems05
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Abstract
This paper presents the first fully integrated gradient-elution liquid chromatography-electrospray ionization (LC-ESI) system on a chip. This chip integrates a pair of high-pressure gradient pumps, a sample injection pump, a passive mixer, a packed separation column, and an ESI nozzle. We also present the successful on-chip separation of protein digests by reverse phase (RP)-LC coupled with on-line mass spectrometer (MS) analysis.
| Item Type: | Book Section |
|---|---|
| Additional Information: | © 2005 IEEE. Reprinted with Permission. Publication Date: 30 Jan.-3 Feb. 2005. Posted online: 2005-07-05. This work is supported in part by NSF ERC Center at Caltech (EEC-9402726) and NIH through grant 5RO1 RR06217-10. |
| Subject Keywords: | chromatography; electron impact ionisation; mass spectroscopic chemical analysis; microfluidics; micropumps; proteins; system-on-chip; ESI nozzle; gradient-LC-ESI system on chip; high-pressure gradient pump; integrated gradient-elution liquid; chromatography-electrospray ionization; on-chip separation; online mass spectrometer analysis; packed separation column; passive mixer; protein analysis; protein digests; reverse phase-LC; sample injection pump |
| Record Number: | CaltechAUTHORS:XIEieeeicmems05 |
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:XIEieeeicmems05 |
| Alternative URL: | http://dx.doi.org/10.1109/MEMSYS.2005.1454045 |
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. |
| ID Code: | 10444 |
| Collection: | CaltechAUTHORS |
| Deposited By: | Kristin Buxton |
| Deposited On: | 06 May 2008 |
| Last Modified: | 26 Dec 2012 10:00 |
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