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Complete gradient-LC-ESI system on a chip for protein analysis

Xie, J. and Shih, J. and Lee, T. D. and Tai, Y. C. (2005) Complete gradient-LC-ESI system on a chip for protein analysis. In: IEEE International Conference on Micro Electro Mechanical Systems, 18th . MEMS 2005. IEEE , Piscataway, NJ, pp. 778-781. ISBN 0-7803-8732-5

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This paper presents the first fully integrated gradient-elution liquid chromatography-electrospray ionization (LC-ESI) system on a chip. This chip integrates a pair of high-pressure gradient pumps, a sample injection pump, a passive mixer, a packed separation column, and an ESI nozzle. We also present the successful on-chip separation of protein digests by reverse phase (RP)-LC coupled with on-line mass spectrometer (MS) analysis.

Item Type:Book Section
Additional Information:© 2005 IEEE. Reprinted with Permission. Publication Date: 30 Jan.-3 Feb. 2005. Posted online: 2005-07-05. This work is supported in part by NSF ERC Center at Caltech (EEC-9402726) and NIH through grant 5RO1 RR06217-10.
Subject Keywords:chromatography; electron impact ionisation; mass spectroscopic chemical analysis; microfluidics; micropumps; proteins; system-on-chip; ESI nozzle; gradient-LC-ESI system on chip; high-pressure gradient pump; integrated gradient-elution liquid; chromatography-electrospray ionization; on-chip separation; online mass spectrometer analysis; packed separation column; passive mixer; protein analysis; protein digests; reverse phase-LC; sample injection pump
Record Number:CaltechAUTHORS:XIEieeeicmems05
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10444
Deposited By: Kristin Buxton
Deposited On:06 May 2008
Last Modified:24 Apr 2015 16:15

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