Siden, E. and Alt, F. W. and Shinefeld, L. and Sato, V. and Baltimore, D. (1981) Synthesis of immunoglobulin µ chain gene products precedes synthesis of light chains during B-lymphocyte development. Proceedings of the National Academy of Sciences of the United States of America, 78 (3). pp. 1823-1827. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:SIDpnas81
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Immunoglobulin (Ig) gene expression has been followed during the later stages of development of the murine fetal liver. Biosynthetic labeling and immunoprecipitation were used to isolate Ig-related polypeptides from fetal and neonatal livers. By examination of the specific immune precipitates, the earliest detectable Ig was shown to consist only of µ heavy chain. At about the time of birth, when light chain synthesis became evident, separation of surface Ig-positive cells from surface Ig-negative cells by using anti-Ig-coated dishes showed that cells lacking surface Ig (pre-B lymphocytes) synthesized only µ chains. Thus, commencement of light chain synthesis was closely coordinated with the appearance of surface Ig. Ig RNA species were examined by electrophoretic fractionation and hybridization with cloned Ig DNA sequences. The sizes and amounts of Ig mRNA were found to correlate with the pattern of µ and light chain protein biosynthesis. µ chain RNA species appeared earlier in gestation than light chain RNA did, and only after birth did light chain sequences reach levels equivalent to those of µ chain. Cell populations enriched in pre-B lymphocytes also contained an excess of µ over light chain mRNA.
|Additional Information:||© 1981 by the National Academy of Sciences. Contributed by David Baltimore, November 24, 1980. We thank Dr. I. Weissman and L. Wysocki for polyvalent anti-mouse Ig serum; D. Levitt and M. Cooper, who communicated their work in progress; Ann Gifford for excellent technical assistance; and Michael Paskind for preparation of the pABAµ-3 DNA fragment. This work was supported by grant MV-34K from the American Cancer Society and Grant CA-14051 from the National Cancer Institute (core grant to Dr. S. Luria). E.S. was a Postdoctoral Fellow of the National Institutes of Health. F.W.A. is a Special Fellow of the Leukemia Society of America. V.S. is a recipient of a National Institutes of Health Research Career Development Award. D.B. is a Research Professor of the American Cancer Society. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||pre-B lymphocytes; fetal liver; immunoglobulin mRNA|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||17 May 2008|
|Last Modified:||26 Dec 2012 10:01|
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