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A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus

Rosenberg, Naomi and Baltimore, David (1976) A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. Journal of Experimental Medicine, 143 (6). pp. 1453-1463. ISSN 0022-1007. http://resolver.caltech.edu/CaltechAUTHORS:ROSjem76

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Abstract

A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction.


Item Type:Article
Additional Information:Copyright © 1976 by Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 9 February 1976. We are grateful to Dr. P.C. Kung for his advice on the use of bovine serum albumin gradients. Supported by grant no. VC-4G from the American Cancer Society, a contract from the Virus Cancer Program, and a grant no. CA-14051 from the National Cancer Institute. [N.R. was a] Postdoctoral fellow of the Massachusetts Division of the American Cancer Society. [D.B. was an] American Cancer Society Professor of Microbiology.
Record Number:CaltechAUTHORS:ROSjem76
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ROSjem76
Alternative URL:http://www.jem.org/cgi/content/abstract/143/6/1453
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:10677
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:03 Jun 2008
Last Modified:26 Dec 2012 10:03

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