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Localization of proteins controlling motility and chemotaxis in Escherichia coli

Ridgway, H. F. and Silverman, M. and Simon, M. I. (1977) Localization of proteins controlling motility and chemotaxis in Escherichia coli. Journal of Bacteriology, 132 (2). pp. 657-665. ISSN 0021-9193. http://resolver.caltech.edu/CaltechAUTHORS:RIDjbact77

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Abstract

Flagellar proteins controlling motility and chemotaxis in Escherichia coli were selectively labeled in vivo with [35S]methionine. This distribution of these proteins in subcellular fractions was examined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and autoradiography. The motA, motB, cheM, and cheD gene products were found to be confined exclusively to the inner cytoplasmic membrane fraction, whereas the cheY, cheW, and cheA (66,000 daltons) polypeptides appeared only in the soluble cytoplasmic fraction. The cheB, cheX, cheZ, and cheA (76,000 daltons) proteins, however, were distributed in both the cytoplasm and the inner membrane fractions. The hag gene product (flagellin) was the only flagellar protein examined that copurified with the outer lipopolysaccharide membrane. Differences in the intracellular locations of the che and mot gene prodcuts presumably reflect the functional attributes of these components.


Item Type:Article
Additional Information:Copyright © 1977 by the American Society for Microbiology. Received for publication 18 August 1977.
Record Number:CaltechAUTHORS:RIDjbact77
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:RIDjbact77
Alternative URL:http://jb.asm.org/cgi/content/abstract/132/2/657
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10688
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:04 Jun 2008
Last Modified:26 Dec 2012 10:03

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