Ridgway, H. F. and Silverman, M. and Simon, M. I. (1977) Localization of proteins controlling motility and chemotaxis in Escherichia coli. Journal of Bacteriology, 132 (2). pp. 657-665. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:RIDjbact77
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Flagellar proteins controlling motility and chemotaxis in Escherichia coli were selectively labeled in vivo with [35S]methionine. This distribution of these proteins in subcellular fractions was examined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and autoradiography. The motA, motB, cheM, and cheD gene products were found to be confined exclusively to the inner cytoplasmic membrane fraction, whereas the cheY, cheW, and cheA (66,000 daltons) polypeptides appeared only in the soluble cytoplasmic fraction. The cheB, cheX, cheZ, and cheA (76,000 daltons) proteins, however, were distributed in both the cytoplasm and the inner membrane fractions. The hag gene product (flagellin) was the only flagellar protein examined that copurified with the outer lipopolysaccharide membrane. Differences in the intracellular locations of the che and mot gene prodcuts presumably reflect the functional attributes of these components.
|Additional Information:||Copyright © 1977 by the American Society for Microbiology. Received for publication 18 August 1977.|
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|Deposited On:||04 Jun 2008|
|Last Modified:||26 Dec 2012 10:03|
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