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Heteroduplex Analysis of the Sequence Relationships Between the Genomes of Kirsten and Harvey Sarcoma Viruses, Their Respective Parental Murine Leukemia Viruses, and the Rat Endogenous 30S RNA

Chien, Yueh-Hsiu and Lai, Michael and Shih, Thomas Y. and Verma, Inder M. and Scolnick, Edward M. and Roy-Burman, Pradip and Davidson, Norman (1979) Heteroduplex Analysis of the Sequence Relationships Between the Genomes of Kirsten and Harvey Sarcoma Viruses, Their Respective Parental Murine Leukemia Viruses, and the Rat Endogenous 30S RNA. Journal of Virology, 31 (3). pp. 752-760. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:CHIjvir79a

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Abstract

The sequence relations between Kirsten murine sarcoma virus (Ki-SV), Harvey murine sarcoma virus (Ha-SV), and a rat endogenous 30S RNA were studied by electron microscope heteroduplex analysis. The sequence relationships between the sarcoma viruses and their respective parental murine leukemia viruses (Kirsten and Moloney murine leukemia viruses), as well as between the two murine leukemia viruses, were also studied. The only observed nonhomology feature of the Kirsten murine leukemia virus/Moloney murine leukemia virus heteroduplexes was a substitution loop with two arms of equal length extending from 1.80 ± 0.18 kilobases (kb) to 2.65 ± 0.27 kb from the 3' end of the RNA. It is believed that this feature lies in the env gene region of the viral genomes. The Ha-SV and Moloney murine leukemia virus genomes (respective lengths, 6.0 and 9.0 kb) were homologous in a 1.0 ± 0.05-kb region at the 3' end and possibly over a 200-nucleotide region at the 5' ends; otherwise, they were nonhomologous. Ha-SV and Ki-SV (length, 7.5 kb) were homologous in the first 4.36 ± 0.37-kb region from the 3' end and in a 0.70 ± 0.15-kb region at the 5' end. In between, there was a nonhomology region, possibly containing a short (0.23-kb) region of partial or total homology. The heteroduplex analysis between rat endogenous 30S RNA and Ki-SV shows that there are mixed regions of sequence homology and nonhomology at both the 5' and 3' ends. However, there is a large (4-kb) region of homology between Ki-SV and the rat 30S RNA in the center of the genomes, with only a small nonhomology hairpin feature. These studies help to define the regions of homology between the Ha-SV and Ki-SV genomes with each other and with the rat endogenous 30S RNA. These regions may be related to the sarcoma genicity of the viruses. In particular, the 0.7-kb region of homology of Ha-SV with Ki-SV at the 5' ends may be related to the formation of a 21,000-dalton phosphoprotein in cells transformed by either virus.


Item Type:Article
Additional Information:Copyright © 1979 by the American Society for Microbiology. Received for publication 28 February 1979. This research was supported by Public Health Service contract N01 CP 43306 from the Division of Cancer Cause and Prevention, National Cancer Institute, to N.D., by Public Health Service grants CA 16561 to I.M.V., CA 21408 to I.M.V., and CA 16113 to M.L. from the National Cancer Institute, and by American Cancer Society research grant VC-176 to M.L.
Record Number:CaltechAUTHORS:CHIjvir79a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:CHIjvir79a
Alternative URL:http://jvi.asm.org/cgi/content/abstract/31/3/752
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10746
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:05 Jun 2008
Last Modified:26 Dec 2012 10:04

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