Haywood, Anne M. and Cramer, Jane Harris and Shoemaker, Nadja Lancaster (1969) Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli. I. Location of "Late" MS2-Specific Ribonucleic Acid Synthesis. Journal of Virology, 4 (4). pp. 364-371. ISSN 0022-538X http://resolver.caltech.edu/CaltechAUTHORS:HAYjvir69
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When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 x g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 x g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg2+. In the presence of high Mg+, MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid.
|Additional Information:||Copyright © 1969 American Society for Microbiology. Received for publication 2 June 1969. We are grateful to Karen Williams and Anne Greenwood for technical assistance. A.M.H. acknowledges ideas from conversations with George Guthrie and Robert Haselkorn that led to an interest in association of MS2-specific proteins and subsequently MS2-specific RNA with "membrane." We are greatly indebted to James and Ellen Strauss for editorial help. This research was supported by Public Health Service grants 1 R01 A106549 and 1 R01 A107788 from the National Institute of Allergy and Infectious Diseases. J.H.C. was supported by National Institutes of Health predoctoral training grant 5 TI GM 724.|
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|Deposited On:||06 Jun 2008|
|Last Modified:||26 Dec 2012 10:04|
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