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Non-AUG Initiation of AGAMOUS mRNA Translation in Arabidopsis thaliana

Riechmann, José Luis and Ito, Toshiro and Meyerowitz, Elliott M. (1999) Non-AUG Initiation of AGAMOUS mRNA Translation in Arabidopsis thaliana. Molecular and Cellular Biology, 19 (12). pp. 8505-8512. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:RIEmcb99

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Abstract

The MADS box organ identity gene AGAMOUS (AG) controls several steps during Arabidopsis thaliana flower development. AG cDNA contains an open reading frame that lacks an ATG triplet to function as the translation initiation codon, and the actual amino terminus of the AG protein remains uncharacterized. We have considered the possibility that AG translation can be initiated at a non-AUG codon. Two possible non-AUG initiation codons, CUG and ACG, are present in the 5' region of AG mRNA preceding the highly conserved MADS box sequence. We prepared a series of AG genomic constructs in which these codons are mutated and assayed their activity in phenotypic rescue experiments by introducing them as transgenes into ag mutant plants. Alteration of the CTG codon to render it unsuitable for acting as a translation initiation site does not affect complementation of the ag-3 mutation in transgenic plants. However, a similar mutation of the downstream ACG codon prevents the rescue of the ag-3 mutant phenotype. Conversely, if an ATG is introduced immediately 5' to the disrupted ACG codon, the resulting construct fully complements the ag-3 mutation. The AG protein synthesized in vitro by initiating translation at the ACG position is active in DNA binding and is of the same size as the AG protein detected from floral tissues, whereas AG polypeptides with additional amino-terminal residues do not appear to bind DNA. These results indicate that translation of AG is initiated exclusively at an ACG codon and prove that non-AUG triplets may be efficiently used as the sole translation initiation site in some plant cellular mRNAs.


Item Type:Article
Additional Information:Copyright © 1999, American Society for Microbiology. Received 11 June 1999/Returned for modification 3 August 1999/Accepted 18 August 1999. We are grateful to Vijaya Rao for helping with Arabidopsis transformations and with plant genotyping and maintenance; to Eva Ziegelhoffer for looking after our plants when needed; and to Catherine Baker, Chiou-Fen Chuang, Jennifer Fletcher, Carolyn Ohno, Doris Wagner, and Eva Ziegelhoffer for valuable comments on the manuscript. This work was supported by National Institutes of Health grant GM45697 to E.M.M.; T.I. was supported by a postdoctoral fellowship from the Japan Society for the Promotion of Science.
Record Number:CaltechAUTHORS:RIEmcb99
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:RIEmcb99
Alternative URL:http://mcb.asm.org/cgi/content/abstract/19/12/8505
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10816
Collection:CaltechAUTHORS
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Deposited On:12 Jun 2008
Last Modified:26 Dec 2012 10:05

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