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Screening procedure for complementation-dependent mutants of vesicular stomatitis virus

Rettenmier, Carl W. and Dumont, Raymonde and Baltimore, David (1975) Screening procedure for complementation-dependent mutants of vesicular stomatitis virus. Journal of Virology, 15 (1). pp. 41-49. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:RETjvir75

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Abstract

To isolate new types of vesicular stomatitis virus (VSV) mutants, a four-stage screen was developed which identifies and characterizes mutants capable of complementing the defect in the VSV temperature-sensitive mutant tsG11. Two types of mutants of VSV, Indiana serotype, have been found by using the screen; they are new temperature-sensitive mutants which are, of necessity, not in complementation group I and mutants which do not produce plaques under conditions of single infection at 31 C (the normal permissive temperature) and are, therefore, called complementation-dependent mutants. The newly isolated, temperature-sensitive mutants fall into three complementation groups, two of which are congruent with known complementation groups; the newly identified group extends to six the number of complementation groups of VSV Indiana. The nature of the complementation-dependent mutants has not been established, but one was shown to not contain a significant deletion in its nucleic acid.


Item Type:Article
Additional Information:Copyright © 1975 by the American Society for Microbiology. Received for publication 11 September 1974. We thank Nora Meuth for excellent technical help. C.W.R. was supported by a National Science Foundation Summer Undergraduate Program; R.D. was supported by a Research Training Fellowship awarded by the International Agency for Research on Cancer; D.B. was an American Cancer Society Research Professor. This work was supported by grant VC-4E from the American Cancer Society.
Record Number:CaltechAUTHORS:RETjvir75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:RETjvir75
Alternative URL:http://jvi.asm.org/cgi/content/abstract/15/1/41
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10820
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:12 Jun 2008
Last Modified:26 Dec 2012 10:05

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