Spector, Deborah H. and Baltimore, David (1975) Polyadenylic acid on poliovirus RNA. II. poly(A) on intracellular RNAs. Journal of Virology, 15 (6). pp. 1418-1431. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:SPEjvir75a
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The content, size, and mechanism of synthesis of 3'-terminal poly(A) on the various intracellular species of poliovirus RNA have been examined. All viral RNA species bound to poly(U) filters and contained RNase-resistant stretches of poly(A) which could be analyzed by electrophoresis in polyacrylamide gels. At 3 h after infection, the poly(A) on virion RNA, relicative intermediate RNA, polyribosomal RNA, and total cytoplasmic 35S RNA was heterogeneous in size with an average length of 75 nucleotides. By 6 h after infection many of the intracellular RNA's had poly(A) of over 150 nucleotides in length, but the poly(A) in virion RNA did not increase in size suggesting that the amount of poly(A) which can be encapsidated is limited. At all times, the double-stranded poliovirus RNA molecules had poly(A) of 150 to 200 nucleotides. Investigation of the kinetics of poly(A) appearance in the replicative intermediate and in finished 35S molecules indicated that poly(A) is the last portion of the 35S RNA to be synthesized; no nascent poly(A) could be detected in the replicative intermediate. Although this result indicates that poliovirus RNA is synthesized 5' leads to 3' like other RNA's, it also suggests that much of the poly(A) found in the replicative intermediate is an artifact possibly arising from the binding of finished 35S RNA molecules to the replicative intermediate during extraction. The addition of poly(A) to 35S RNA molecules was not sensitive to guanidene.
|Additional Information:||Copyright © 1975 by the American Society for Microbiology. Received for publication 10 March 1975. The work was supported by Public Health Service grants CA-14051 (from the National Cancer Institute) and AI-08383 (from the National Institute for Allergy and Infectious Diseases). D.H.S. was a predoctoral fellow of the National Science Foundation for part of the work. D.B. is an American Cancer Society Research professor.|
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|Deposited On:||15 Jun 2008|
|Last Modified:||26 Dec 2012 10:06|
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