Speck, Nancy A. and Baltimore, David (1987) Six distinct nuclear factors interact with the 75-base-pair repeat of the Moloney murine leukemia virus enhancer. Molecular and Cellular Biology, 7 (3). pp. 1101-1110. ISSN 0270-7306. http://resolver.caltech.edu/CaltechAUTHORS:SPEmcb87
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Binding sites for six distinct nuclear factors on the 75-base-pair repeat of the Moloney murine leukemia virus enhancer have been identified by an electrophoretic mobility shift assay combined with methylation interference. Three of these factors, found in WEHI 231 nuclear extracts, which we have named LVa, LVb, and LVc (for leukemia virus factors a, b, and c) have not been previously identified. Nuclear factors that bind to the conserved simian virus 40 corelike motif, the NF-1 motif, and the glucocorticoid response element were also detected. Testing of multiple cell lines showed that most factors appeared ubiquitous, except that the NF-1 binding factor was found neither in nuclear extracts from MEL cells nor in the embryonal carcinoma cell lines PCC4 and F9, and core-binding factor was relatively depleted from MEL and F9 nuclear extracts.
|Additional Information:||Copyright © 1987 by the American Society for Microbiology. Received 3 November 1986/Accepted 11 December 1986. We thank D. Weaver, L. Staudt, and R. Sen for their help in generating the library of extracts and column fractions described and, along with Yoav Citri, for helpful advice. We also thank Ginger Pierce for quick and efficient typing of the manuscript. N.S. was supported by postdoctoral fellowship A106713 from the National Institutes of Health. This work was supported by grant IM-355R from the American Cancer Society.|
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|Deposited On:||15 Jun 2008|
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