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Ribonucleic Acud Polymerase in Virions of Newcastle Disease Virus: Comparison with the Vesicular Stomatitis Virus Polymerase

Huang, Alice S. and Baltimore, David and Bratt, Michael A. (1971) Ribonucleic Acud Polymerase in Virions of Newcastle Disease Virus: Comparison with the Vesicular Stomatitis Virus Polymerase. Journal of Virology, 7 (3). pp. 389-394. ISSN 0022-538X. http://resolver.caltech.edu/CaltechAUTHORS:HUAjvir71

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Abstract

The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymerase was less than 3% that of the VSV polymerase. Product RNA species from the NDV and VSV polymerase reactions annealed specifically to the homologous virion RNA species. Transcriptive intermediates containing product RNA attached to the respective virion RNA could be identified in both systems.


Item Type:Article
Additional Information:Copyright © 1971 by the American Society for Microbiology. Received for publication 4 January 1971. This investigation was supported by Public Health Service grants AI-08388 and AI-80367 from the National Institute of Allergy and Infectious Diseases and by American Cancer Society Research grant E 512. D.B. is a Faculty Research Awardee of the American Cancer Society. We gratefully acknowledge the technical assistance of Judith Rice and Hildegard Keary.
Record Number:CaltechAUTHORS:HUAjvir71
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HUAjvir71
Alternative URL:http://jvi.asm.org/cgi/content/abstract/7/3/389
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10911
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:16 Jun 2008
Last Modified:26 Dec 2012 10:06

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