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Polypeptide Secretion from the Isolated Parietovisceral Ganglion of Aplysia californica

Arch, S. (1972) Polypeptide Secretion from the Isolated Parietovisceral Ganglion of Aplysia californica. Journal of General Physiology, 59 (1). pp. 47-59. ISSN 0022-1295. http://resolver.caltech.edu/CaltechAUTHORS:ARCjgp72a

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Abstract

In vitro studies of the secretory behavior of the parietovisceral ganglion in Aplysia californica were performed. The aim of these studies was to investigate the release of polypeptides in response to depolarizing stimuli, and, in particular, to determine if a specific polypeptide known to induce egg laying in the intact animal is secreted into the bathing medium. During continuous perfusion of a ganglion preincubated in leucine-3H the application of either high-potassium medium or a burst of electrical stimuli (via the pleurovisceral connective nerve) evoked a marked increase in the amount of trichloroacetic acid (TCA)-precipitable radioactivity recovered in the perfusate. Enhanced release could be detected within 80 sec of the initial exposure to high potassium; however, incubation of a ganglion in calcium-free media before the application of high-potassium medium abolished the increase of precipitable radioactivity. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of perfusate samples revealed a significant change in the polypeptide species washed from the ganglion during high-potassium depolarization. Bioassays confirmed that egg laying is induced when high-potassium medium used to bathe a ganglion is injected into a recipient animal. These and other results permit the conclusion that the bulk of the polypeptide material secreted from the ganglion in response to depolarization is a specific neurohormone produced by two identified cell clusters, the so-called bag cells.


Item Type:Article
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http://www.jgp.org/cgi/content/abstract/59/1/47PublisherUNSPECIFIED
Additional Information:Copyright © 1972 by The Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Submitted on May 14, 1971. I wish to thank Dr. Felix Strumwasser for his advice and support during these studies. Helpful comments on the manuscript were provided by Doctors Strumwasser, Arnold Eskin, and David L. Wilson. Miss Shelly Rempel and Mr. James Gilliam rendered expert technical assistance. This research was supported by grants from the United States Public Health Service (NB 07071), National Aeronautics and Space Administration (NGR 05-002-031), and the American Heart Association (69-1023).
Funders:
Funding AgencyGrant Number
Public Health ServiceNB 07071
NASANGR 05-002-031
American Heart Association69-1023
Record Number:CaltechAUTHORS:ARCjgp72a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:ARCjgp72a
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:11210
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:23 Jul 2008 23:53
Last Modified:26 Dec 2012 10:10

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