Arch, S. (1972) Biosynthesis of the Egg-Laying Hormone (ELH) in the Bag Cell Neurons of Aplysia californica. Journal of General Physiology, 60 (1). pp. 102-119. ISSN 0022-1295. http://resolver.caltech.edu/CaltechAUTHORS:ARCjgp72b
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Biosynthesis of the egg-laying hormone in the bag cell neurons of Aplysia californica was studied. Bag cells were incubated with leucine-3H in vitro for 30 min and rinsed for variable periods of time in a chase medium. The distribution of incorporated label among proteins within the cells was assayed by electrophoresis of an homogenate on sodium dodecyl sulfate polyacrylamide gels. Results from rinse times shorter than 30 min revealed that the predominant synthetic product is a 25,000 dalton protein. With longer rinse times, this species was reduced and two species of lower molecular weight became prominent. This redistribution of radioactivity was quantitative and was not prevented by inhibition of protein synthesis during the rinse. A 10°C reduction in temperature (from 15°C) blocked the redistribution. These data are interpreted to indicate that the 25,000 dalton molecule is a precursor which is cleaved enzymatically to yield two lower molecular weight products. One product is a 12,000 dalton molecule which remains in the cell bodies. The other is a molecule of <10,000 daltons which is exported from the somata into the neurohemal regions of the connective tissue. Perfusion of these regions with high [K+] medium results in the release of this product into the medium. It is concluded that this product is the 6000 dalton egg-laying hormone (ELH).
|Additional Information:||Copyright © 1972 by The Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Submitted on February 7, 1972. I wish to thank Dr. Felix Strumwasser for his advice and support during these studies. Helpful comments on the manuscript were made by Doctors Strumwasser and David L. Wilson. Ms. Shelly Rempel rendered expert technical assistance. This research was supported by a postdoctoral fellowship from the National Institutes of Health (N.I.G.M.S., 1F02 GM50884-01) and grants from the United States Public Health Service (NB-07071) and the National Aeronautics and Space Administration (NGR05-002-031).|
|Usage Policy:||RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.|
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