Lau, Arthur L. Y. and Chan, Sunney I. (1975) Alamethicin-mediated fusion of lecithin vesicles. Proceedings of the National Academy of Sciences of the United States of America, 72 (6). pp. 2170-2174. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:LAUpnas75
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It was recently shown that alamethicin greatly facilitates the fusion of small, sonicated, lecithin bilayer vesicles. In the present work the details of this fusion process have been followed by monitoring the inner and outer choline methyl signals separately by proton magnetic resonance spectroscopy. It is shown that during the alamethicin-induced fusion some of the antibiotic molecules become translocated from the extravesicular aqueous medium into the enclosed intravesicular space, and these alamethicin molecules were found to affect the choline methyl signals from the inner half of the bilayer only. No evidence was obtained for transmembrane coupling of the two halves of the bilayer in the presence of alamethicin or for any effects that might be construed as due to incorporation of alamethicin molecules into the hydrophobic core of the bilayer.
|Additional Information:||Copyright © 1975 by the National Academy of Sciences. Communicated by John D. Baldeschwieler, March 19, 1975. We thank Dr. G. B. Whitfield, Jr., of the Upjohn Co., Kalamazoo, Mich., for his generous gift of two 25-mg samples of alamethicin which made this and other related studies possible. This work was supported by Grant no. GM-14523-09 from the National Institute of General Medical Sciences, U.S. Public Health Service. A.L.Y.L. is a Danforth Predoctoral Fellow, 1971-1975. This is Contribution no. 5049.|
|Subject Keywords:||nuclear magnetic resonance, model membranes, antibiotics, ion transport across membranes, voltage gateable ion channels|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||21 Dec 2005|
|Last Modified:||26 Dec 2012 08:42|
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