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Alamethicin-mediated fusion of lecithin vesicles

Lau, Arthur L. Y. and Chan, Sunney I. (1975) Alamethicin-mediated fusion of lecithin vesicles. Proceedings of the National Academy of Sciences of the United States of America, 72 (6). pp. 2170-2174. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:LAUpnas75

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Abstract

It was recently shown that alamethicin greatly facilitates the fusion of small, sonicated, lecithin bilayer vesicles. In the present work the details of this fusion process have been followed by monitoring the inner and outer choline methyl signals separately by proton magnetic resonance spectroscopy. It is shown that during the alamethicin-induced fusion some of the antibiotic molecules become translocated from the extravesicular aqueous medium into the enclosed intravesicular space, and these alamethicin molecules were found to affect the choline methyl signals from the inner half of the bilayer only. No evidence was obtained for transmembrane coupling of the two halves of the bilayer in the presence of alamethicin or for any effects that might be construed as due to incorporation of alamethicin molecules into the hydrophobic core of the bilayer.


Item Type:Article
Additional Information:Copyright © 1975 by the National Academy of Sciences. Communicated by John D. Baldeschwieler, March 19, 1975. We thank Dr. G. B. Whitfield, Jr., of the Upjohn Co., Kalamazoo, Mich., for his generous gift of two 25-mg samples of alamethicin which made this and other related studies possible. This work was supported by Grant no. GM-14523-09 from the National Institute of General Medical Sciences, U.S. Public Health Service. A.L.Y.L. is a Danforth Predoctoral Fellow, 1971-1975. This is Contribution no. 5049.
Subject Keywords:nuclear magnetic resonance, model membranes, antibiotics, ion transport across membranes, voltage gateable ion channels
Record Number:CaltechAUTHORS:LAUpnas75
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:LAUpnas75
Alternative URL:http://www.pnas.org/cgi/content/abstract/72/6/2170
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1126
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Dec 2005
Last Modified:14 Nov 2014 19:18

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