Shimoda, Y. and Hurley, J. B. and Miller, W. H. (1984) Rod light response augmented by active phosphodiesterase. Proceedings of the National Academy of Sciences of the United States of America, 81 (2). pp. 616-619. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:SHIpnas84
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Light activates rod outer segment (ROS) phosphodiesterase (PDEase), as shown by previous biochemical and physiological studies. We have further investigated the role of PDEase in this system by injecting trypsin-activated PDEase, purified from bovine ROS, into single ROS of the isolated retina of the toad Bufo marinus. Injection of about 300 molecules of activated PDEase in darkness is without immediate detectable effect, as measured by intracellular membrane-voltage recording. The effect of the activated PDEase injections only becomes evident after illumination. The light response is augmented; kinetics of repolarization are slowed. We conclude that this augmentation of the light-dependent hyperpolarization results from the hydrolysis of endogenous cyclic GMP caused by injected PDEase. These results provide evidence that PDEase affects light-dependent channels of the vertebrate scotopic photoreceptors but do not specify whether the effects are exercised for the initial hyperpolarizing phase of the receptor potential and for the recovery phase or only for the recovery phase.
|Additional Information:||© 1984 by the National Academy of Sciences. Communicated by Floyd Ratliff, September 19, 1983. We are indebted to Professor Lubert Stryer (Stanford University School of Medicine) and Professor E.N. Pugh, Jr. (University of Pennsylvania) for painstaking reviews, to Professor Melvin Simon (California Institute of Technology) for his support, and to Rosemary Hopkins for technical assistance. This work was supported in part by Public Health Service Grants AI-19296 (M.I. Simon, California Institute of Technology) and EY-03196 (W.H.M.) and by fellowships from Fight for Sight, Inc., New York City (Y.S.) and the Alexander B. Cox Memorial Fund, Yale Medical School (Y.S.). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.|
|Subject Keywords:||sensory transduction; vertebrate scotopic receptors; receptor potential; light-dependent channels|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||06 Aug 2008 01:26|
|Last Modified:||26 Dec 2012 10:12|
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