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Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation

Britten, Roy J. and Davidson, Eric H. (1976) Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation. Proceedings of the National Academy of Sciences of the United States of America, 73 (2). pp. 415-419. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:BRIpnas76

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Abstract

The rate of appearance of duplex DNA renaturation, measured with single strand specific nuclease, deviates significantly from a second order reaction. Measurements reported in paper I of this series indicate an inhibition in the rate of reassociation of single strand tails on partially reassociated molecules by a factor of at least two. Equations are derived that describe the observed form of reassociation kinetics as measured with hydroxyapatite and with single strand specific nuclease. The free parameter that describes the extent of inhibition of nucleation with single strand tails in these equations has been evaluated by least squares methods and agrees with the experimentally measured value.


Item Type:Article
Related URLs:
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http://www.pnas.org/content/73/2/415.abstractPublisherUNSPECIFIED
Additional Information:© 1976 by the National Academy of Sciences. Contributed by Roy J. Britten, November 26, 1975. We thank Drs. Norman Davidson, Michael Smith, and Lynn Klotz for their critical reviews of a draft of this paper. This work was supported by NIH Grants HD-05753 and GM-20927 and by NSF Grant GB-33441.
Funders:
Funding AgencyGrant Number
National Institutes of HealthHD-05753
National Institutes of HealthGM-20927
National Science FoundationGB-33441
Subject Keywords:renaturation; nucleation inhibition; single strand ends
Record Number:CaltechAUTHORS:BRIpnas76
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BRIpnas76
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11337
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Aug 2008 23:46
Last Modified:14 Nov 2014 19:20

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