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Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation

Britten, Roy J. and Davidson, Eric H. (1976) Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation. Proceedings of the National Academy of Sciences of the United States of America, 73 (2). pp. 415-419. ISSN 0027-8424. PMCID PMC335919.

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The rate of appearance of duplex DNA renaturation, measured with single strand specific nuclease, deviates significantly from a second order reaction. Measurements reported in paper I of this series indicate an inhibition in the rate of reassociation of single strand tails on partially reassociated molecules by a factor of at least two. Equations are derived that describe the observed form of reassociation kinetics as measured with hydroxyapatite and with single strand specific nuclease. The free parameter that describes the extent of inhibition of nucleation with single strand tails in these equations has been evaluated by least squares methods and agrees with the experimentally measured value.

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Additional Information:© 1976 by the National Academy of Sciences. Contributed by Roy J. Britten, November 26, 1975. We thank Drs. Norman Davidson, Michael Smith, and Lynn Klotz for their critical reviews of a draft of this paper. This work was supported by NIH Grants HD-05753 and GM-20927 and by NSF Grant GB-33441.
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Subject Keywords:renaturation; nucleation inhibition; single strand ends
PubMed Central ID:PMC335919
Record Number:CaltechAUTHORS:BRIpnas76
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11337
Deposited By: Tony Diaz
Deposited On:08 Aug 2008 23:46
Last Modified:17 Dec 2015 01:16

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