Emerson, Suzanne U. and Simon, Melvin I. (1971) Variation in the Primary Structure of Bacillus subtilis Flagellins. Journal of Bacteriology, 106 (3). pp. 949-954. ISSN 0021-9193 http://resolver.caltech.edu/CaltechAUTHORS:EMEjbact71
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The flagella derived from 18 strains Bacillus subtilis were tested for their reaction with antiflagellar filament antibody and antiflagellin antibody. On the basis of their reactivity, at least five serologically distinct classes could be identified. Peptide map analysis of tryptic digests of the subunit proteins were consistent with the immunochemical analysis. Large differences in sequence existed among proteins of the different classes; proteins within an antigenic group differed by only a few peptides. Furthermore, 9 of the 27 tryptic peptides resolved were common to flagellin proteins from all the classes examined. The relationship between antigenic specificity, variability in peptide pattern, and the conformation of the flagellin protein are discussed.
|Additional Information:||Copyright © 1971 American Society for Microbiology. Received for publication 4 March 1971. This work was supported by a grant from the National Science Foundation (no. GB-15655). Taken from a dissertation submitted by Suzanne U. Emerson, in June 1970, to the University of California, San Diego, in partial fulfillment of the requirement for the Ph.D. degree.|
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|Deposited On:||14 Aug 2008 23:35|
|Last Modified:||26 Dec 2012 10:14|
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