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Isolation of a new high molecular weight protein associated with desmin and vimentin filaments from avian embryonic skeletal muscle

Breckler, Jennifer and Lazarides, Elias (1982) Isolation of a new high molecular weight protein associated with desmin and vimentin filaments from avian embryonic skeletal muscle. Journal of Cell Biology, 92 (3). pp. 795-806. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:BREjcb82

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Abstract

Filaments with a diameter of 80-120 Å have been prepared from 14-d-old chick embryonic skeletal muscle, using a physiological salt solution and gel filtration chromatography. The filaments obtained are composed of the two known muscle intermediate-filament proteins, vimentin and desmin, as well as the vimentin- and desmin-associated high molecular weight protein, synemin (230,000 mol. wt). In addition, they contain a previously unidentified high molecular weight protein (280,000 mol wt) which differs from synemin by isoelectric point, molecular weight, and immunological reactivity. Immunofluorescence on cultured myogenic cells,using antisera to the 280,000-dalton polypeptide, has revealed that this protein has the same spatial distribution as desmin, vimentin, and synemin in both early myotubes, where it associates with cytoplasmic filaments, and late in myotubes, where it is associated with myofibril Z lines. Examination by immunofluorescence of frozen sections of developing embryonic skeletal muscle reveals a gradual diminution in the presence of the 280,000-dalton protein. The 280,000-dalton protein is undetectable in adult skeletal and smooth muscle, as shown by immunofluorescence and immunoautoradiography. In chick embryonic fibroblasts grown in tissue culture, only a subpopulation of the cells is reactive with antibodies to the 280,000-dalton protein even though all these cells contain vimentin. In the reactive cells, vimentin and the 280,000-dalton polypeptide exhibit an indistinguishable cytoplasmic filamentous network, which aggregates into filamentious bundles when the cells are exposed to colcemid. These results suggest that this newly identified high molecular weight protein is closely associated with intermediate filaments containing either vimentin alone or vimentin, desmin and synemin. The expression of this protein appears to be developmentally regulated and does not appear to parallel the expression of any of the other three intermediate-filament proteins. The absence of the 280,000-dalton polypeptide in adult muscle cells and its gradual reduction during development implies that is probably not required for the maintenance of Z-disk structure after the assembly of the sarcomere.


Item Type:Article
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http://www.jcb.org/cgi/content/abstract/92/3/795PublisherUNSPECIFIED
Additional Information:© 1982 by The Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 15 June 1981, and in revised form 21 September 1981. We thank B.L. Granger, R.H. Gomer, J. Gelles, and Dr. E. Repasky for their help in various aspects of this work and Drs. E. Repasky, B. Granger, D. Gard, I. Sandoval, and C. Colaco for their many valuable comments on the manuscript. We also thank Ilga Lielausis for expert technical assistance. This work was supported by grants from the National Institutes of Health (NIH) (PHS-GM 06965), the Muscular Dystrophy Association of America, the National Science Foundation, and a Biomedical Research Support Grant from U.S. Public Health Service. J. Breckler was supported by a postdoctoral fellowship from the American Heart Association Greater Los Angeles Affiliate. E. Lazarides is a recipient of a NIH Research Career Development Award.
Funders:
Funding AgencyGrant Number
National Institutes of HealthPHS-GM 06965
Muscular Dystrophy Association of AmericaUNSPECIFIED
National Science FoundationUNSPECIFIED
U.S. Public Health ServiceUNSPECIFIED
American Heart Association, Greater Los Angeles AffiliateUNSPECIFIED
National Institutes of Health, Research Career Development AwardUNSPECIFIED
Record Number:CaltechAUTHORS:BREjcb82
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BREjcb82
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:11472
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:26 Aug 2008 18:38
Last Modified:26 Dec 2012 10:14

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