Vermot, Julien and Fraser, Scott E. and Liebling, Michael (2008) Fast fluorescence microscopy for imaging the dynamics of embryonic development. HFSP Journal, 2 (3). pp. 143-155. ISSN 1955-2068 http://resolver.caltech.edu/CaltechAUTHORS:VERhfspj08
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Live imaging has gained a pivotal role in developmental biology since it increasingly allows real-time observation of cell behavior in intact organisms. Microscopes that can capture the dynamics of ever-faster biological events, fluorescent markers optimal for in vivo imaging, and, finally, adapted reconstruction and analysis programs to complete data flow all contribute to this success. Focusing on temporal resolution, we discuss how fast imaging can be achieved with minimal prejudice to spatial resolution, photon count, or to reliably and automatically analyze images. In particular, we show how integrated approaches to imaging that combine bright fluorescent probes, fast microscopes, and custom post-processing techniques can address the kinetics of biological systems at multiple scales. Finally, we discuss remaining challenges and opportunities for further advances in this field.
|Additional Information:||© 2008 HFSP Publishing. Received 14 January 2008; accepted 19 March 2008; published 13 May 2008. We thank Elaine Bearer for providing us with the squid giant axon data used in Fig. 4. We thank Willy Supatto, Thai Truong, and members of the Biological Imaging Center, Beckman Institute at Caltech for discussions and comments. M.L. received support from the Swiss National Science Foundation (Fellowship PA002-111433) and J.V. from the Human Frontier Science Program (HFSP). This work was also supported by a NIH/NICHD grant (PO1HD037105).|
|Subject Keywords:||biomedical optical imaging, cell motility, fluorescence, image reconstruction, image resolution, medical image processing, optical microscopy|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Archive Administrator|
|Deposited On:||31 Aug 2008 05:13|
|Last Modified:||26 Dec 2012 10:15|
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