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The influence of non-neuronal cells on catecholamine and acetylcholine synthesis and accumulation in cultures of dissociated sympathetic neurons

Patterson, Paul H. and Chun, Linda L. Y. (1974) The influence of non-neuronal cells on catecholamine and acetylcholine synthesis and accumulation in cultures of dissociated sympathetic neurons. Proceedings of the National Academy of Sciences of the United States of America, 71 (9). pp. 3607-3610. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:PATpnas74

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Abstract

The effects of several non-neuronal cell types on neurotransmitter synthesis in cultures of dissociated sympathetic neurons from the new-born rat were studied. Acetylcholine synthesis from radioactive choline was increased 100- to 1000-fold in the presence of non-neuronal cells from sympathetic ganglia. This increase was roughly dependent on the number of ganglionic non-neuronal cells present. The effect did not appear to be due to an increased plating efficiency of neurons, since the non-neuronal cells were capable of increasing acetylcholine synthesis after only 48-hr contact with neurons that had been previously grown without non-neuronal cells for 2 weeks. C6 rat glioma cells were also able to stimulate acetylcholine synthesis, but 3T3 mouse fibroblast cells had little or no effect. None of the non-neuronal cell types synthesized detectable acetylcholine in the absence of the neurons. The ganglionic non-neuronal cells had no significant effect on catecholamine synthesis (which occurs in the absence of non-neuronal cells).


Item Type:Article
Additional Information:Copyright © 1974 by the National Academy of Sciences. Communicated by Stephen W. Kuffler, July 1, 1974. We acknowledge the able assistance of K. Fischer with the cell lines and D. McDowell with the culturing. This work was supported by American Heart Association Grant 73-877 and U.S. Public Health Service Grant iROl NS 11027-01 from the National Institute of Neurological Diseases and Stroke (NINDS). P.H.P. is a Research Career Development Awardee of NINDS (1K04 NS 70806-01) and L.L.Y.C. is a U.S. Public Health Service trainee (TO1 NS 05731-02).
Subject Keywords:cell culture, cell-cell interaction
Record Number:CaltechAUTHORS:PATpnas74
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:PATpnas74
Alternative URL:http://www.pnas.org/cgi/content/abstract/71/9/3607
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1161
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:30 Dec 2005
Last Modified:26 Dec 2012 08:43

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