Meares, Claude F. and Sundberg, Michael W. and Baldeschwieler, John D. (1972) Perturbed angular correlation study of a haptenic molecule. Proceedings of the National Academy of Sciences of the United States of America, 69 (12). pp. 3718-3722. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:MEApnas72
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The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of (111)In is strongly perturbed when the I-p-nitrophenylethylenediaminetetraacetate chelate of (111)In(3+) is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average association constant between the antibody and the labeled chelate has been estimated from perturbed angular correlation measurements; this value is compared to the results of equilibrium dialysis. These experiments provide good evidence that information concerning macromolecular behavior can be obtained from perturbed angular correlation experiments that use chemically specific labels.
|Additional Information:||Copyright © 1972 by the National Academy of Sciences. Contributed by John D. Baldeschwieler, October 5, 1972. We thank Dr. G. Rosenquist, Dr. J. H. Freed, and Prof. H. O. McDevitt for their advice and help. This work was supported by the National Institutes of Health (GM-14752), the National Science Foundation (GP4924), and the Center for Materials Research, Stanford University. C. F. M. was supported by a National Science Foundation Graduate Fellowship (1968-1972).|
|Subject Keywords:||rotational label, anti-Dnp, radioactive, (111)In|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||30 Dec 2005|
|Last Modified:||26 Dec 2012 08:43|
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