CaltechAUTHORS
  A Caltech Library Service

Isolation of a peptide in guinea pig liver homogenate and its turnover of leucine

Borsook, Henry and Deasy, Clara L. and Haagen-Smit, A. J. and Keighley, Geoffrey and Lowy, Peter H. (1948) Isolation of a peptide in guinea pig liver homogenate and its turnover of leucine. Journal of Biological Chemistry, 174 (3). pp. 1041-1042. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:BORjbc48b

[img]
Preview
PDF - Published Version
See Usage Policy.

100Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:BORjbc48b

Abstract

Leucine was synthesized with C14 in the carboxyl group. 10 mg. of the radioactive amino acid (DL) and 0.66 gm. (wet weight) of guinea pig liver homogenate were added to a reaction mixture containing 1.3 per cent of an amino acid mixture corresponding to the composition of casein and 0.005 M fumarate, all in a final volume of 4 ml. of isotonic saline solution(1) at pH 7.4. The reaction was carried out under oxygen for 6 hours at 38°.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jbc.org/cgi/reprint/174/3/1041PublisherUNSPECIFIED
Additional Information:© 1948 American Society of Biological Chemists. Received for publication, May 14, 1948. The authors are indebted to and wish to thank Dr. S. Moore and Dr. W.H. Stein of The Rockefeller Institute for Medical Research for their generosity and courtesy in giving the details of their method of starch chromatography and ninhydrin calorimetry prior to publication. This work is part of that done under contract with the Office of Naval Research, United States Navy Department. The C14 used in this investigation was supplied by the Monsanto Chemical Company, Clinton Laboratories, and was obtained on allocation from the United States Atomic Energy Commission.
Funders:
Funding AgencyGrant Number
Office of Naval ResearchUNSPECIFIED
Atomic Energy CommissionUNSPECIFIED
Record Number:CaltechAUTHORS:BORjbc48b
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BORjbc48b
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11789
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Oct 2008 19:00
Last Modified:26 Dec 2012 10:20

Repository Staff Only: item control page