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The uptake in vitro of C14-labeled glycine, L-leucine, and L-lysine by different components of guinea pig liver homogenate

Borsook, Henry and Deasy, Clara L. and Haagen-Smit, A. J. and Keighley, Geoffrey and Lowy, Peter H. (1950) The uptake in vitro of C14-labeled glycine, L-leucine, and L-lysine by different components of guinea pig liver homogenate. Journal of Biological Chemistry, 184 (2). pp. 529-543. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:BORjbc50a

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Abstract

We have reported (1) that L-lysine labeled with C14 can be incorporated into the proteins of guinea pig liver homogenate under two different conditions. In the one case the enzyme used was the whole homogenate, the optimum pH was near 6.2, there was an obligatory requirement of calcium, and the incorporation was independent of oxygen. This set of conditions is designated below as the “acid calcium” condition. In the other case the enzyme system was the precipitate obtained by centrifuging the homogenate diluted 15-fold with Ringer’s solution at 2500 X g, the optimum pH was near to 7.3, the reaction was accelerated a little by calcium but the presence of calcium was not obligatory, and the incorporation was a little less under nitrogen than under oxygen. This set of conditions is designated below as the “alkaline” condition.


Item Type:Article
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http://www.jbc.org/content/vol184/issue2/index.shtmlPublisherUNSPECIFIED
Additional Information:© 1950 American Society of Biological Chemists. Received for publication, November 21, 1949. This work was carried out under the joint sponsorship of the United States Atomic Energy Commission and the Office of Naval Research. The C14 used in this investigation was supplied by the Carbide and Carbon Chemicals Corporation, Oak Ridge, Tennessee, and obtained on allocation from the United States Atomic Energy Commission.
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Atomic Energy CommissionUNSPECIFIED
Office of Naval ResearchUNSPECIFIED
Record Number:CaltechAUTHORS:BORjbc50a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BORjbc50a
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11812
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Oct 2008 17:27
Last Modified:26 Dec 2012 10:21

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