Wren, J. J. and Mitchell, Herschel K. (1959) Extraction Methods and an Investigation of Drosophila Lipids. Journal of Biological Chemistry, 234 (11). pp. 2823-2828. ISSN 0021-9258. http://resolver.caltech.edu/CaltechAUTHORS:WREjbc59
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In earlier work (8) we extracted lipids from dried, macerated Drosophila melanogaster with ether, but later, working with larger quantities of undried flies, we found that most of the phospholipids were autolyzed. Kates' studies (2) led him to suggest n-propanol or isopropanol for lipid extraction (isopropanol was his later choice (6, 7)). Attempting to meet the requirements discussed above, we developed a new and relatively simple method of extraction employing n-propanol (9), or chloroformmethanol (2:1). The latter proved to be a more useful solvent. The method will be described in detail below, with results of an examination of Drosophila lipids.
|Additional Information:||Copyright © 1959 by the American Society for Biochemistry and Molecular Biology. (Received for publication, December 17, 1958) This investigation was supported in part by grants from the National Science Foundation.|
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|Deposited On:||02 Oct 2008 07:04|
|Last Modified:||26 Dec 2012 10:21|
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