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Migrating neural crest cells in the trunk of the avian embryo are multipotent

Fraser, Scott E. and Bronner, Marianne E. (1991) Migrating neural crest cells in the trunk of the avian embryo are multipotent. Development, 112 (4). pp. 913-922. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:FRAdev91

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Abstract

Trunk neural crest cells migrate extensively and give rise to diverse cell types, including cells of the sensory and autonomic nervous systems. Previously, we demonstrated that many premigratory trunk neural crest cells give rise to descendants with distinct phenotypes in multiple neural crest derivatives. The results are consistent with the idea that neural crest cells are multipotent prior to their emigration from the neural tube and become restricted in phenotype after leaving the neural tube either during their migration or at their sites of localization. Here, we test the developmental potential of migrating trunk neural crest cells by microinjecting a vital dye, lysinated rhodamine dextran (LRD), into individual cells as they migrate through the somite. By two days after injection, the LRD-labelled clones contained from 2 to 67 cells, which were distributed unilaterally in all embryos. Most clones were confined to a single segment, though a few contributed to sympathetic ganglia over two segments. A majority of the clones gave rise to cells in multiple neural crest derivatives. Individual migrating neural crest cells gave rise to both sensory and sympathetic neurons (neurofilament-positive), as well as cells with the morphological characteristics of Schwann cells, and other non-neuronal cells (both neurofilament-negative). Even those clones contributing to only one neural crest derivative often contained both neurofilament-positive and neurofilament-negative cells. Our data demonstrate that migrating trunk neural crest cells can be multipotent, giving rise to cells in multiple neural crest derivatives, and contributing to both neuronal and non-neuronal elements within a given derivative. Thus, restriction of neural crest cell fate must occur relatively late in migration or at the final sites of neural crest cell localization.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/112/4/913PublisherUNSPECIFIED
Additional Information:Copyright © 1991 by Company of Biologists. (Accepted 9 April 1991) We thank Kristin Bruk and Mary Flowers for excellent technical assistance and Drs Andres Collazo and Jonathan Ivins for helpful comments on the manuscript. This work was supported by USPHS (HD-25138).
Funders:
Funding AgencyGrant Number
U.S. Public Health ServiceHD-25138
Subject Keywords:cell lineage, vital dye, microinjection, neural crest, migration, avian embryo
Record Number:CaltechAUTHORS:FRAdev91
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:FRAdev91
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11827
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:02 Oct 2008 20:22
Last Modified:26 Dec 2012 10:21

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