CaltechAUTHORS
  A Caltech Library Service

The substrate in peptic synthesis of protein

Borsook, Henry and MacFadyen, Douglas A. and Wasteneys, Hardolph (1930) The substrate in peptic synthesis of protein. Journal of General Physiology, 13 (3). pp. 295-306. ISSN 0022-1295. http://resolver.caltech.edu/CaltechAUTHORS:BORjgp30a

[img]
Preview
PDF - Published Version
Creative Commons Attribution Non-commercial Share Alike.

656Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:BORjgp30a

Abstract

Experiments are described in which it was observed that the yield of protein that can be synthesized by pepsin from a given peptic digest is highest when the hydrolyzing action of the pepsin is stopped as soon as all the protein has disappeared from the solution; and that the longer the digest is permitted to contain active enzyme the more the yield diminishes. 2. Exposure of the digest to a hydrogen ion concentration of pH 1.6 in the absence of active enzyme, does not cause a diminution in the amount of protein which can be synthesized from that digest. 3. Synthesis can be effected also in concentrated solutions of isolated fractions of a peptic digest, i.e. of proteose and of peptone. The yields are approximately the same as in similar concentrations of the whole digest, though the proteins so synthesized differ in some respects from those obtained from the whole digest. 4. The cessation of synthesis in any one digest is due to the attainment of equilibrium and not to the complete utilization of available synthesizeable material. The amount of the equilibrium yield, on the other hand, is dependent on the amount of synthesizeable material in the digest. 5. These observations are taken to show that the synthesizeability of a given mixture of protein cleavage products by pepsin depends upon its possession of a special complex in these products. This complex appears as a result of the primary hydrolysis of the protein molecule by pepsin and is decomposed in the slow secondary hydrolysis which ensues as digestion is prolonged.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jgp.org/cgi/content/abstract/13/3/295PublisherUNSPECIFIED
Additional Information:© 1930 Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Accepted for publication, October 16, 1929.
Record Number:CaltechAUTHORS:BORjgp30a
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BORjgp30a
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:11831
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Oct 2008 04:34
Last Modified:26 Dec 2012 10:21

Repository Staff Only: item control page