Borsook, Henry and Dubnoff, Jacob W. (1947) Ornithine-catalyzed urea formation in liver homogenate. Journal of Biological Chemistry, 169 (2). pp. 461-462. ISSN 0021-9258 http://resolver.caltech.edu/CaltechAUTHORS:BORjbc47c
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Heretofore attempts to obtain synthesis of urea from ammonia (and carbon dioxide) in cell-free extracts have been unsuccessful. We have found the reaction to proceed in guinea pig liver homogenate. The following is the reaction mixture which has given the highest yields obtained so far: L-ornithine (0.00075 M), ammonia (0.0025 M), L-glutamate (0.01 M), oxalacetate (0.005 M), ATP (0.00025 M), and 0.33 gm. of homogenized liver in a final volume of 3.5 ml. The following are typical increases in urea over the blank observed in 1 hour, in micrograms: ornithine + ammonia 0, glutamate + oxalacetate 0, glutamate + oxalacetate + ornithine 49, glutamate + oxalacetate + ammonia 105, glutamate + oxalacetate + ornithine + ammonia 317, glutamate + ocalacetate + ornithine + ammonia (without ATP) 150.
|Additional Information:||© 1947 American Society of Biological Chemists. Received for publication, May 5, 1947. This work is a part of that done under contract with the Office of Naval Research, United States Navy Department.|
|Usage Policy:||No commercial reproduction, distribution, display or performance rights in this work are provided.|
|Deposited By:||Tony Diaz|
|Deposited On:||09 Oct 2008 01:35|
|Last Modified:||26 Dec 2012 10:22|
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