van Harreveld, A. and Malhotra, S. K. (1966) Demonstration of Extracellular Space by Freeze-Drying in the Cerebellar Molecular Layer. Journal of Cell Science, 1 (2). pp. 223-228. ISSN 0021-9533 http://resolver.caltech.edu/CaltechAUTHORS:HARjcb66
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In electron micrographs of the molecular layer of the mouse cerebellum frozen within 30 sec of circulatory arrest and subsequently dried at -79 °C an appreciable extracellular space was found between the axons of the granular cells. Tight junctions were regularly observed between pre- and postsynaptic structures and the enveloping glia cells. In micrographs of cerebellum frozen 8 min after decapitation the space between the axons was absent and tight junctions between the nerve fibres were almost exclusively encountered. The extracellular space of asphyxiated and non-asphyxiated tissue in electron micrographs of frozen-dried material is similar to the space in comparable tissues treated by freeze-substitution. These observations suggest that there is an appreciable amount of extracellular material in oxygenated, living tissue which is taken up by cellular elements during asphyxiation.
|Additional Information:||Copyright © 1966 by Company of Biologists. Submitted on November 2, 1965. This investigation was supported, in part, by a grant from the National Science Foundation (G-21531).|
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|Deposited On:||07 Oct 2008 22:33|
|Last Modified:||26 Dec 2012 10:22|
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