CaltechAUTHORS
  A Caltech Library Service

The Magnitude of the Extracellular Space in Electron Micrographs of Superficial and Deep Regions of the Cerebral Cortex

van Harreveld, A. and Steiner, Jana (1970) The Magnitude of the Extracellular Space in Electron Micrographs of Superficial and Deep Regions of the Cerebral Cortex. Journal of Cell Science, 6 (3). pp. 793-805. ISSN 0021-9533. http://resolver.caltech.edu/CaltechAUTHORS:HARjcs70

[img]
Preview
PDF - Published Version
See Usage Policy.

3448Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:HARjcs70

Abstract

Electron micrographs (EMs) of central nervous tissue perfused with glutaraldehyde and post-fixed with osmium tetroxide exhibit more extracellular space in superficial than in deep regions of the brain. It was postulated that this is due to the availability to the surface tissue of the cerebrospinal fluid in the subarachnoidal space. It was assumed that in addition to the extracellular material this fluid is taken up by dendritic elements in the surface layer of the cortex during the glutaraldehyde perfusion. Indeed, dendritic structures in EMs of the surface layer of glutaraldehyde-perfused and OsO4-post-fixed cortex exhibited a more pronounced swelling than similar structures in deeper cortical regions, where only extracellular material was available to the dendrites. The extracellular space in such preparations consisted of narrow slits surrounding the tissue elements. These slits were formed during the post-fixation since they were not present in freeze-substituted, glutaraldehyde-perfused tissue, which showed a paucity of extracellular material. The slits were in general wider in the superficial than in the deep cortical tissue. That this difference is related to the amount of material taken up by the dendrites during the glutaraldehyde perfusion was supported by experiments in which the exposed cortical surface was flooded with Ringer's solution during the glutaraldehyde perfusion. This resulted in a ballooning of dendritic elements in the cortical surface due to the availability of an unlimited amount of fluid on the cortex. Post-fixed material of these flooded cortices yielded EMs with unusually wide extracellular slits around the tissue elements.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jcs.biologists.org/cgi/content/abstract/6/3/793PublisherUNSPECIFIED
Additional Information:Copyright © 1970 by Company of Biologists. Submitted on July 21, 1969. This investigation was supported by a grant from the National Science Foundation (GB 6698) and from the Barber Fund.
Funders:
Funding AgencyGrant Number
National Science FoundationGB 6698
Barber FundUNSPECIFIED
Record Number:CaltechAUTHORS:HARjcs70
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:HARjcs70
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11864
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:07 Oct 2008 23:32
Last Modified:26 Dec 2012 10:22

Repository Staff Only: item control page