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Competence, specification and induction of Pax-3 in the trigeminal placode

Baker, Clare V. H. and Stark, Michael R. and Marcelle, Christophe and Bronner, Marianne E. (1999) Competence, specification and induction of Pax-3 in the trigeminal placode. Development, 126 (1). pp. 147-156. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:BAKdev99

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Abstract

Placodes are discrete regions of thickened ectoderm that contribute extensively to the peripheral nervous system in the vertebrate head. The paired-domain transcription factor Pax-3 is an early molecular marker for the avian ophthalmic trigeminal (opV) placode, which forms sensory neurons in the ophthalmic lobe of the trigeminal ganglion. Here, we use collagen gel cultures and heterotopic quail-chick grafts to examine the competence, specification and induction of Pax-3 in the opV placode. At the 3-somite stage, the whole head ectoderm rostral to the first somite is competent to express Pax-3 when grafted to the opV placode region, though competence is rapidly lost thereafter in otic-level ectoderm. Pax-3 specification in presumptive opV placode ectoderm occurs by the 8-somite stage, concomitant with robust Pax-3 expression. From the 8-somite stage onwards, significant numbers of cells are committed to express Pax-3. The entire length of the neural tube has the ability to induce Pax-3 expression in competent head ectoderm and the inductive interaction is direct. We propose a detailed model for Pax-3 induction in the opV placode.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dev.biologists.org/cgi/content/abstract/126/1/147PublisherUNSPECIFIED
Additional Information:Copyright © 1999 by Company of Biologists. Accepted 19 October; published on WWW 3 December 1998. We are very grateful to Dr Andy Groves for discussion and helpful comments on the manuscript. This work was supported by USPHS NS34671 and NS36585 (M.B.F.), and by Human Frontier Science Program Fellowship LT-63/96 and American Heart Association Fellowship 1171-FI1 (C.V.H.B.).
Funders:
Funding AgencyGrant Number
Public Health ServiceNS34671
Public Health ServiceNS36585
Human Frontier Science ProgramLT-63/96
American Heart Association1171-FI1
Subject Keywords:Pax-3, Induction, Trigeminal, Placode, Cranial ganglia, Chick
Record Number:CaltechAUTHORS:BAKdev99
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:BAKdev99
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11890
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:08 Oct 2008 23:17
Last Modified:26 Dec 2012 10:23

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