Bonner, James and Widholm, Jack (1967) Molecular complementarity between nuclear DNA and organ-specific chromosomal RNA. Proceedings of the National Academy of Sciences of the United States of America, 57 (5). pp. 1379-1385. ISSN 0027-8424 http://resolver.caltech.edu/CaltechAUTHORS:BONpnas67
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Abstract
We have shown earlier (1) that chromosomes contain RNA which is associated with the chromosomal proteins, the majority of which are histories. In pea chromatin these RNA molecules are approximately 40 nucleotides in length, contain about 27 mole per cent dihydrouridylic acid, and are associated with chromosomal DNA in such a way that they are protected from attack by RNase. As a first step in the study of the role of this class of RNA we have investigated the characteristics of its hybridization with nuclear DNA. It is shown below that the RNA component of protein-bound chromosomal RNA is heterogeneous and that it hybridizes with 5 per cent of nuclear DNA, a ratio approximating the ratio of this RNA to DNA in native chromatin.
| Item Type: | Article |
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| Additional Information: | © 1967 by the National Academy of Sciences. Communicated March 29, 1967. We gratefully acknowledge the counsel and support of our colleagues Professors Norman Davidson, Jerome Vinograd, and Ru-chich C. Huang, Dr. Keiji Marushige, and Michael E. Dahmus and Douglas Fambrough. We acknowledge with pleasure the skillful assistance of Georgia Lau, Katherine Ching-Mei Ku, and Ludia Brown. Report of work supported in part by the Herman Frasch Foundation, and by the U.S. Public Health Service, grant GM-AM-13,762. |
| Record Number: | CaltechAUTHORS:BONpnas67 |
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:BONpnas67 |
| Usage Policy: | You are granted permission for individual, educational, research and non-commercial reproduction, distribution, display and performance of this work in any format. |
| Metadata Review: | All Records > Caltech Library Services |
| ID Code: | 11908 |
| Collection: | CaltechAUTHORS |
| Deposited By: | Tony Diaz |
| Deposited On: | 17 Oct 2008 19:57 |
| Last Modified: | 17 Oct 2008 19:57 |
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