Wilson, David L. (1971) Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia. Journal of General Physiology, 57 (1). pp. 26-40. ISSN 0022-1295 http://resolver.caltech.edu/CaltechAUTHORS:WILjgp71
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Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells.
|Additional Information:||RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 2 May 1970. I would like to acknowledge a Helen Hay Whitney Foundation postdoctoral fellowship. This research was aided by grants from the U.S. Public Health Service (NB07071), NASA (NGR-05-002-031), and the American Heart Association, Inc. (69-1023). I would like to thank Felix Strumwasser for helpful discussions and advice on techniques and for a critical reading of this paper. I am indebted to Shelly Rempel for technical assistance and to James Gilliam for the design and construction of a number of useful devices.|
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