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Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia

Wilson, David L. (1971) Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia. Journal of General Physiology, 57 (1). pp. 26-40. ISSN 0022-1295. http://resolver.caltech.edu/CaltechAUTHORS:WILjgp71

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Abstract

Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintillation counting of the gels. Experiments showed that: (a) a number of proteins were being synthesized in abundance in the nerve cells; (b) different, identified neurons showed reproducibly different labeling patterns in the gels; (c) cells R2 and R15, which showed different distributions of radioactivity in the gels, had similar staining patterns; and (d) there was significant incorporation into material of high (>75,000) molecular weight in most of the cells.


Item Type:Article
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http://www.jgp.org/cgi/content/abstract/57/1/26PublisherUNSPECIFIED
Additional Information:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 2 May 1970. I would like to acknowledge a Helen Hay Whitney Foundation postdoctoral fellowship. This research was aided by grants from the U.S. Public Health Service (NB07071), NASA (NGR-05-002-031), and the American Heart Association, Inc. (69-1023). I would like to thank Felix Strumwasser for helpful discussions and advice on techniques and for a critical reading of this paper. I am indebted to Shelly Rempel for technical assistance and to James Gilliam for the design and construction of a number of useful devices.
Funders:
Funding AgencyGrant Number
Helen Hay Whitney FoundationUNSPECIFIED
US Public Health ServiceNB07071
NASANGR-05-002-031
American Heart Association69-1023
Record Number:CaltechAUTHORS:WILjgp71
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WILjgp71
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:11923
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:09 Oct 2008 23:20
Last Modified:26 Dec 2012 10:23

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