David, Charles N. and Easterbrook, Kenneth (1971) Ferritin in the fungus Phycomyces. Journal of Cell Biology, 48 (1). pp. 15-28. ISSN 0021-9525 http://resolver.caltech.edu/CaltechAUTHORS:DAVjcb71
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The iron-protein ferritin has been purified from mycelium, sporangiophores, and spores of the fungus Phycomyces blakesleeanus. It has a protein-to-iron ratio of 5, a sedimentation coefficient of 55S, a buoyant density in CsCl of 1.82 g/cm3, and the characteristic morphology of ferritin in the electron microscope. Apoferritin prepared from Phycomyces ferritin has a sedimentation coefficient of 18S and consists of subunits of molecular weight 25,000. In the cytoplasm of Phycomyces, ferritin is located on the surface of lipid droplets (0.5–2.0 µ in diameter) where it forms crystalline monolayers which are conspicuous in electron micrographs of sporangiophore thin-sections. Ferritin is found in all developmental stages of Phycomyces but is concentrated in spores. The level of ferritin iron is regulated by the iron level in the growth medium, a 50-fold increase occurring on iron-supplemented medium.
|Additional Information:||© 1971 Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Submitted on November 14, 1968. Revised on July 13, 1970. The authors thank Dr. Max Delbrück for initiating their interest in Phycomyces ferritin and for criticism during the completion of this work. The electron micrograph in Fig. 7 was kindly supplied by Dr. E. William Goodell. This work was supported by National Science Foundation Grant GB-4642. C.N. David had a predoctoral fellowship from United States Public Health Service Trainee Grant No. GM-00086.|
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|Deposited By:||Tony Diaz|
|Deposited On:||21 Oct 2008 02:58|
|Last Modified:||26 Dec 2012 10:24|
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