Loh, Dennis and Ross, Alonzo H. and Hale, Arthur H. and Baltimore, David and Eisen, Herman N. (1979) Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes. Journal of Experimental Medicine, 150 (5). pp. 1067-1074. ISSN 0022-1007 http://resolver.caltech.edu/CaltechAUTHORS:LOHjem79
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Synthetic phospholipid vesicles (liposomes) containing the purified glycoprotein (G) of vesicular stomatitis virus (VSV) and solubilized membrane proteins from cells of the appropriate H-2 haplotype elicited H-2-restricted cytotoxic T lymphocytes (CTL) that lysed VSV-infected target cells. The CTL were elicited by intact liposomes, not by released components. Thus, when spleen cells from VSV-primed H-2d X H- 2b hybrid mice were stimulated with liposomes having G protein + membrane proteins from cells with one of the parental H-2 haplotypes, the resulting CTL lysed only VSV-infected target cells with that parent's H-2 type. This result argues against the view that T cells in general recognize only processed antigenic fragments on macrophages. Moreover, liposomes were only effective when G protein and cell membrane proteins were included in the same vesicles. This result suggests that for effective interaction with CTL precursors the antigen (G protein) and products of the H-2 complex must be closer to each other than 600-1,000 angstrom, the diameter of the lipid vesicles used in this study.
|Additional Information:||Copyright © 1979 by the Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 29 May 1979. Supported by Research grant CA-15472 and Center grant CA-14051 to the Massachusetts Institute of Technology, Center for Cancer Research, from the National Cancer Institute, and by a research grant from the American Cancer Society (IM-161), and the North Carolina United Way and Forsythe Cancer Service. Alonzo H. Ross is a Damon Runyon-Walter Winchell Cancer Fund Fellow (DRG-144-FT). David Baltimore is a Research Professor of the American Cancer Society.|
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