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Inhibition of movement of trition-demembranated sea-urchin sperm flagella by Mg2+, ATP4-, ADP and P1

Okuno, Makoto and Brokaw, C. J. (1979) Inhibition of movement of trition-demembranated sea-urchin sperm flagella by Mg2+, ATP4-, ADP and P1. Journal of Cell Science, 38 . pp. 105-123. ISSN 0021-9533. http://resolver.caltech.edu/CaltechAUTHORS:OKUjcs79

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Abstract

Three clinical patterns of inhibition of MgATP2--activated flagellar motility have been found by measuring the motility of Triton-demembranated sea-urchin spermatozoa beating with their heads attached to a glass surface. Inhibition of beat frequency by the reaction products, ADP and Pi, is competitive with the normal substrate, MgATP2-, and the inhibitory effects are similar to a reduction in MgATP2- concentration. Inhibition of beat frequency by ATP4- is competitive with MgATP2, but is accompanied by an inhibition of bending, as measured by the angle between the straight regions on either side of a bend, which is not seen when MgATP2- concentration is reduced. Inhibition of beat frequency by Mg2+ is not competitive with MgATP2-, and is accompanied by an increase in bend angle, so that there is no change in the rate of sliding between flagellar tubules. These differences suggest unexpected complexity of dynein ATPase action in flagella. The beat frequencies of both swimming and attached spermatozoa show a linear double reciprocal dependence on MgATP2- concentration, with identical slopes. The calculated sliding velocities between tubules also give linear relationships, but the slopes are different, suggesting that beat frequency may be the more fundamental dependent variable in this system.


Item Type:Article
Related URLs:
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http://jcs.biologists.org/cgi/content/abstract/38/1/105PublisherUNSPECIFIED
Additional Information:Copyright © 1979 by Company of Biologists. (Received % January 1979) We thank Tom Simonick for extensive assistance with these experiments, and the United States Public Health Service for support by grant No. GM-18711 awarded by the National Institute of General Medical Sciences and by Biomedical Research Support Grant No. RR 07003 awarded by the Division of Research Resources.
Funders:
Funding AgencyGrant Number
National Institute of General Medical SciencesGM-18711
Public Health ServiceRR 07003
Record Number:CaltechAUTHORS:OKUjcs79
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:OKUjcs79
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:12074
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Deposited On:21 Oct 2008 23:15
Last Modified:26 Dec 2012 10:26

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