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Study of carbonic anhydrase using perturbed angular correlations of gamma radiation

Meares, Claude F. and Bryant, Robert G. and Baldeschwieler, John D. and Shirley, David A. (1969) Study of carbonic anhydrase using perturbed angular correlations of gamma radiation. Proceedings of the National Academy of Sciences of the United States of America, 64 (4). pp. 1155-1161. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:MEApnas69

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Abstract

The angular correlation of the 150-247 kev gamma-ray cascade of (111m)Cd is strongly perturbed when this nucleus is bound to the enzyme carbonic anhydrase. A comparison of the perturbed angular correlation for the apoenzyme with that for native carbonic anhydrase confirms that the (111m)Cd binds at the active region of the enzyme. These results provide good evidence that the perturbed angular correlation reflects the effective molecular rotational correlation time at the metal binding site, and that this radioactive nucleus can be used as a rotational tracer to label biological macromolecules. The qualitative dependence of the perturbed angular correlation of the (111m)Cd cascade on the molecular rotational correlation time at the metal binding site is illustrated using a cadmium-complex solution at various temperatures.


Item Type:Article
Additional Information:Copyright © 1969 by the National Academy of Sciences. Communicated by Harden M. McConnell, September 15, 1969. The assistance of Mrs. Winifred Heppler, Mr. George Gabor, Mrs. Penny Fink, and Dr. Lloyd Robinson is gratefully acknowledged.
Record Number:CaltechAUTHORS:MEApnas69
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:MEApnas69
Alternative URL:http://www.pnas.org/cgi/content/abstract/64/4/1155
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:1212
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Jan 2006
Last Modified:14 Nov 2014 19:18

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