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Properties of an antiserum against native dynein 1 from sea urchin sperm flagella

Ogawa, K. and Asai, D. J. and Brokaw, C. J. (1977) Properties of an antiserum against native dynein 1 from sea urchin sperm flagella. Journal of Cell Biology, 73 (1). pp. 182-192. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:OGAjcb77

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Abstract

Effects of an antiserum against native dynein 1 from sperm flagella of the sea urchin Strongylocentrotus purpuratus were compared with effects of an antiserum previously obtained against an ATPase-active tryptic fragment (fragment 1A) of dynein 1 from sperm flagella of the sea urchin, Anthocidaris crassispina. Both antisera precipitate dynein 1 and do not precipitate dynein 2. Only the fragment 1A antiserum precipitates fragment 1A and produces a measurable inhibition of dynein 1 ATPase activity. Both antisera inhibit the movement and the movement- coupled ATP dephosphorylation of reactivated spermatozoa. The inhibition of movement by the antiserum against dynein 1 is much less than by the antiserum against fragment 1A, suggesting that a specific interference with the active ATPase site may be required for effective inhibition of movement. Both antisera reduce the bend angle as well as the beat frequency of reactivated S. purpuratus spermatozoa, suggesting that the bend angle may depend on the activity of the dynein arms which generate active sliding.


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http://jcb.rupress.org/cgi/content/abstract/73/1/182PublisherUNSPECIFIED
Additional Information:© 1977 Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 28 September 1976, and in revised form 7 December 1976. We thank Tom Simonick and Eileen Roy for able assistance with portions of this work; Professor Ray Owen for immunology laboratory facilities and advice; and the U.S. Public Health Service for support provided by National Institutes of Health research grant GM 18711.
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Funding AgencyGrant Number
National Institutes of HealthGM 18711
Record Number:CaltechAUTHORS:OGAjcb77
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:OGAjcb77
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:12127
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:01 Nov 2008 02:02
Last Modified:26 Dec 2012 10:27

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