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Control of erythroid differentiation: asynchronous expression of the anion transporter and the peripheral components of the membrane skeleton in AEV- and S13-transformed cells

Woods, Catherine M. and Boyer, Brigitte and Vogt, Peter K. and Lazarides, Elias (1986) Control of erythroid differentiation: asynchronous expression of the anion transporter and the peripheral components of the membrane skeleton in AEV- and S13-transformed cells. Journal of Cell Biology, 103 (5). pp. 1789-1798. ISSN 0021-9525. http://resolver.caltech.edu/CaltechAUTHORS:WOOjcb86

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Abstract

Chicken erythroblasts transformed with avian erythroblastosis virus or S13 virus provide suitable model systems with which to analyze the maturation of immature erythroblasts into erythrocytes. The transformed cells are blocked in differentiation at around the colony-forming unit- erythroid stage of development but can be induced to differentiate in vitro. Analysis of the expression and assembly of components of the membrane skeleton indicates that these cells simultaneously synthesize alpha-spectrin, beta-spectrin, ankyrin, and protein 4.1 at levels that are comparable to those of mature erythroblasts. However, they do not express any detectable amounts of anion transporter. The peripheral membrane skeleton components assemble transiently and are subsequently rapidly catabolized, resulting in 20-40-fold lower steady-state levels than are found in maturing erythrocytes. Upon spontaneous or chemically induced terminal differentiation of these cells expression of the anion transporter is initiated with a concommitant increase in the steady- state levels of the peripheral membrane-skeletal components. These results suggest that during erythropoiesis, expression of the peripheral components of the membrane skeleton is initiated earlier than that of the anion transporter. Furthermore, they point a key role for the anion transporter in conferring long-term stability to the assembled erythroid membrane skeleton during terminal differentiation.


Item Type:Article
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http://jcb.rupress.org/cgi/content/abstract/103/5/1789PublisherUNSPECIFIED
Additional Information:Copyright © 1986 by The Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 12 August 1986. We thank Dr, John Cox and Jeff Stack for advice on Northern analysis and for comments on the manuscript, and Ann Rafferty and Michele Lamka for excellent technical assistance. This work was supported by grants from the National Institutes of Health (AGO6078A-01) and National Science Foundation (DCB 8215658 A02) to E. Lazarides and by CA13213 and CA29777 from the National Institutes of Health to P.K. Vogt. C.M. Woods was supported by a postdoctoral fellowship from Muscular Dystrophy Associations of America; B. Boyer was a Centre National de la Recherche Scientifique fellow and was also supported by a Research Training fellowship from the International Agency for Research on Cancer.
Funders:
Funding AgencyGrant Number
National Institutes of HealthAGO6078A-01
National Science FoundationDCB 8215658 A02
National Institutes of HealthCA13213
National Institutes of HealthCA29777
Muscular Dystrophy AssociationUNSPECIFIED
Centre National de la Recherche ScientifiqueUNSPECIFIED
International Agency for Research on CancerUNSPECIFIED
Record Number:CaltechAUTHORS:WOOjcb86
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WOOjcb86
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:12206
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:29 Oct 2008 04:02
Last Modified:26 Dec 2012 10:28

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