Dusenberry, David B. and Uretz, Robert B. (1972) The orientation of DNA within 80-Angstrom chromatin fibers. Journal of Cell Biology, 52 (3). pp. 639-647. ISSN 0021-9525 http://resolver.caltech.edu/CaltechAUTHORS:DUSjcb72
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Squashing salivary glands of Chironomus thummi larvae, Amblystoma tigrinum erythrocytes, or Spirostromum frequently results in stretched chromatin having highly oriented DNA as determined by polarized fluorescence microscopy of acridine orange-stained preparations. The examination of such material from C. thummi in the electron microscope indicates that the individual chromatin fibers have an average thickness of 80 A as is usually found in embedded and sectioned material. It is thus concluded that the DNA lies nearly parallel to the axis of these chromatin fibers. Detailed calculations of the polarization expected from various models of DNA packing are contained in an appendix.
|Additional Information:||Copyright © 1972 by Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Submitted on June 11, 1970. Revised on November 8, 1971. The authors wish to thank G. Grofman for valuable assistance with electron microscopy and related procedures. Dr. Dusenbery was a recipient of a United States Public Health Service traineeship, GM 780. This research was supported by United States Public Health Service Research Grant No. CA 02739 from the National Cancer Institute.|
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