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The colorimetric determination of hexoses with carbazole

Holzman, George and MacAllister, Robert V. and Niemann, Carl (1947) The colorimetric determination of hexoses with carbazole. Journal of Biological Chemistry, 171 (1). pp. 27-35. ISSN 0021-9258.

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The optimum conditions for the calorimetric estimation of hexoses by reaction with carbazole in hot sulfuric acid solution have been determined and a convenient procedure, giving results with a precision of 2 to 5 per cent in the range of 50 to 150 γ of glucose, is described. The colors obtained with glucose, galactose, fructose, and mannose are not sufficiently distinctive to allow their ready differentiation and identification by spectral measurements. The significance of the ultraviolet spectra of heated and unheated sulfuric acid solutions of hexoses to the problem of estimation and identification of hexoses is discussed.

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Additional Information:Copyright © 1947 by American Society of Biological Chemists. (Received for publication, July 16, 1947) Gates and Crellin Laboratories of Chemistry, Contribution No. 1137.
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Gates and Crellin Laboratories of Chemistry1137
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Deposited On:15 Dec 2008 18:19
Last Modified:26 Dec 2012 10:36

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